Mixers and jets for X-ray spectroscopy

Our lab has a long-standing and fruitful collaboration with Drs. Daniel DePonte and Thomas Kroll of the SLAC National Accelerator Laboratory, a national lab on the Stanford campus. This collaboration grants us unique access to multi-billion dollar SLAC facilities, including integration of microfluidic devices and kinetics assays of our design with SLAC’s Synchrotron and Free Electron Laser X-ray facilities. We create novel microfluidic systems for X-ray absorption and emission spectroscopy studies of reaction kinetics. These mixers and jets have low sample consumption and offer very fast mixing. For example, we developed a three-dimensional hydrodynamic focusing of a reagent enzyme stream down to 500 nm diameter. We tailor the downstream channel to execute a post-mixing, rapid expansion (and deceleration) of the flow to enable high SNR reaction kinetics studies with temporal resolutions from tens of microseconds to several seconds.

Reference

Diego A. Huyke, Ashwin Ramachandran, Diego I. Oyarzun, Thomas Kroll, Daniel P. DePonte, and Juan G. Santiago. "On the competition between mixing rate and uniformity in a coaxial hydrodynamic focusing mixer" Analytica Chimica Acta 1103 (2020): 1-10.

Diego A. Huyke, Alexandre S. Avaro, Thomas Kroll, and Juan G. Santiago. "Stream lamination and rapid mixing in a microfluidic jet for X-ray spectroscopy studies" Flow 3 (2023): E25.